Rat intestinal fatty acid binding protein (iFABP) elisa kit is very easy to use - Database & Sql Blog Articles

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Rat intestinal fatty acid binding protein (iFABP) elisa kit

Basic Information

Abbreviated Chinese name: rat intestinal fatty acid binding protein (iFABP) elisa kit

English abbreviation: iFABP ELISA Kit Specification: 48/96T

Chinese name alias: rat intestinal fatty acid binding protein (iFABP) detection kit

High sensitivity, high efficiency, original imported antibody, uniform adsorption, good adsorption, high recovery and high reliability, ineffective return and replacement, the company provides free testing services, all kinds of ELISA kits are complete. reliable quality.

Product Name: Rat intestinal fatty acid binding protein (iFABP) elisa assay kit instruction manual

English abbreviation: iFABP ELISA Kit

Specification: 96T/48T

Transport conditions: 2-8 ° C low temperature transport, low temperature transport with dry ice or ice pack

Rat intestinal fatty acid binding protein (iFABP) elisa kit product use

For the determination of the content or activity in human serum, plasma, tissue and related liquid samples.

Detection species: large mouse, human, guinea pig, rabbit, pig, dog, cattle and sheep, chicken and duck ELISA kit and other species.

Tips: Please read the instructions thoroughly before use. The enzyme-linked immunosorbent kit is based on the principle of classical enzyme-linked sandwich technology and can only be used for research purposes and should not be used for medical diagnosis.

Rat intestinal fatty acid binding protein (iFABP) elisa assay kit operation

Description

Rat intestinal fatty acid binding protein (iFABP) elisa assay kit instructions for use of specimens

1. The specimens should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 °C, but repeated freezing and thawing should be avoided.

2. Cannot detect samples containing NaN3, inhibiting horseradish peroxidase (HRP) activity by NaN3

Washing method

1. Manual washing method: suck (not touch the wall) or pry off the liquid in the microplate; lay several layers of absorbent paper on the test bench, and take the microplate with the microplate down several times; the recommended washing buffer Inject at least 0.3 ml into the well and soak for 1-2 minutes. Repeat this process several times as needed.

2. Automatic washing: If there is an automatic washing machine, it should be used in the formal experiment after skilled use.

Buy rat intestinal fatty acid binding protein (iFABP) elisa assay kit instruction manual customer notice

1. Liquid specimens (cell culture supernatant, tissue homogenate, serum, plasma, urine, pleural effusion, ascites, cerebrospinal fluid, etc.);

2. Sample storage: Please check as soon as possible, save at 2-8 °C for one day; take longer to freeze (-20 °C or -70 °C). If you need to collect samples periodically, please pack the samples in time and store them at -20 °C or -70 °C.

3. Please tell us in advance: the species, sample size, indicators to be tested and other special requirements of your sample.

Rat intestinal fatty acid binding protein (iFABP) elisa assay kit Instructions for use:

1. When using the kit for the first time, the user should centrifuge the various reagent tubes for a few minutes to concentrate the reagents on the bottom of the tube.

2. Leave one hole for each experiment as a blanking zero hole. This hole is not added with any reagents, only the final substrate solution and stop solution. Use this hole to adjust the OD value to zero when measuring.

3. To prevent evaporation of the sample, place the reaction plate in a closed box covered with a damp cloth during the test, and add or cover the plate.

4. Unused plate or reagent, please store at 2-8 °C. Standard, biotinylated antibody working solution, horseradish peroxidase labeled avidin working solution should be used according to the required amount. Do not reuse diluted standards, biotinylated antibody working solutions, or horseradish peroxidase-labeled avidin working solutions.

5. It is recommended to set the double hole measurement when testing the sample to ensure the accuracy of the test results.

Purchase the company's elisa kit, enjoy zero-cost shipping, and improve after-sales. In addition, you can enjoy the authoritative enzyme-linked immunoassay service, skilled operators, and sophisticated laboratory equipment to ensure that each experimental data is true and reliable. The ELISA kits sold by the company are of quality assurance! Technical guidance is provided throughout the sale and after sale, and there are quality problems, which can be returned free of charge!