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EL-C1600N100013-B
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SMD aluminum electrolytic capacitor
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ELISA operation common problem "sample" solution
ELISA methods are widely used in various antigen and antibody assays. However, there are many influencing factors in the ELISA assay, and there are certain technical requirements in the operation. In addition to the normal reaction, sometimes some wrong results are often found in the detection process. The main causes of ELISA error results are: 1 specimen factor; 2 reagent factor; 3 operational factors. The following is a detailed analysis of the problems in the “adding†operation of the elisa kit.
Shanghai Yuanmu Biological Co., Ltd. specializes in the production and supply of kit products: double-anti-sand sandwich ELISA, ELISA kit, human ELISA kit, enzyme-linked detection kit, domestic imported reagent, elisa kit, ELISA kit , rat ELISA kit, mouse ELISA kit, apoptosis related factor kit, interleukin ELISA kit, VIP ELISA kit and other laboratory products, product quality, quality assurance.
step
Possible Causes
Solution
Material selection
Select a good quality test reagent (see the ELISA kit purchase experience summary), and equilibrate the reagent at room temperature for 30-60 minutes before the operation.
plus
kind
1.
If the serum or plasma specimens are not well separated, the sample is loaded;
2.
In manual operation, too many sample plates cause too long waiting time before loading into the incubator.
(
Especially when the indoor temperature is high
)
;
3.
The enzyme spills out of the well when the specimen is added and the enzyme reagent is added.
1.
The specimen is serum: it is best to store the blood naturally.
1-2
After hours, reuse
3000rmp
Centrifugation
15
Minutes; specimens are plasma: blood specimen collection tubes containing anticoagulants must be used, and blood collection must be reversed immediately after blood collection
5-10
Once, after a period of time,
3000rpm
Centrifugation
15
Minutes; if detected within a few days, can be placed
2-8 ° C
In the refrigerator, if it is to be stored, it is placed
-20 ° C
Inside the low temperature refrigerator.
2.
Put it into the incubator in time after loading.
3.
After adding the enzyme reagent, use a blotting paper to gently blot and dry on the surface of the microplate.
4.
If adopted
AT
Or other automatic sample loading, the best choice
FAME
Or other post-processing instruments plus enzyme reagents.
5.
When there are many specimens, please operate in batches.