Biological safety cabinet use operation process - Database & Sql Blog Articles

BSC-1300

The IIA2 Biosafety Cabinet is a horizontal airflow biosafety product developed based on the protection principle of biosafety cabinets. Provides basic protection for operators, the environment and products during operation. Each safety BSC-1300IIA2 biosafety cabinet is factory tested separately before delivery to meet professional performance requirements. The BSC-1300IIA2 biosafety cabinet protects the operator, the laboratory environment, and the laboratory materials from exposure to the above operations when operating infectious materials such as primary cultures, bacterial strains, and diagnostic specimens. Infectious aerosols and spills that may be produced during the process. Before starting the machine, the front glass window should be closed and the ultraviolet lamp should be turned on for 30 minutes. At this time, the operator and other personnel should avoid it to avoid injury. The fan is then operated for 5 minutes while the exhaust (drain) valve at the bottom of the cabinet is closed to ensure stable airflow to the cabinet.

Before the second work, first turn off the UV lamp, then the operator should adjust the chair to a comfortable height, make sure that your face is above the front glass window, and the arm enters the safety cabinet for about 1 minute before you can operate. The safety cabinet restores airflow and allows the airflow to "flush" the microbes on the arm surface. When the operator's arm is placed flat on the working compartment front grille, some airflow will flow directly to the working area instead of entering the negative pressure channel. This problem can be solved by lifting the arm slightly. Therefore, the front grille should not be placed with any items such as test records, waste plastic packaging, various equipment, etc., to ensure that the airflow smoothly enters the negative pressure passage through the grille.

When the three safety cabinets are in use, the front glass plate should be in working condition. It is recommended that the opening height be slightly less than 20 cm. 4. The equipment and equipment used in the safety cabinet should be minimized, and the air circulation on the rear and sides should not be blocked. There should be a gap between items such as equipment and equipment.

5. Bunsen burners are not allowed in the safety cabinet. A small electronic "furnace" can be used with a power of less than 1000W. A sterile single inoculation loop is recommended.

When the operator is working, the arm should not frequently enter and exit the working area of ​​the safety cabinet. Otherwise, the airflow will be disrupted, the protective air curtain will be damaged, and the personnel and the environment will cause injury and pollution.

7. When operating in a safety cabinet, measures should be taken to avoid cross-contamination. For example, open test tubes and bottles should not be placed vertically. Operators should use a lid to cover the culture dish and tissue culture dish (the vertical straw collection container should not be used in the biosafety cabinet). To reduce the downward flow directly impacting the open sterile surface, the cap and cap should be reattached as soon as possible after the operation is completed.

After the end of the eight operations, about 10 minutes is the self-cleaning time of the work area, after which the fan is turned off and the ultraviolet lamp is sterilized for 30 minutes, at which time the personnel leave. At the end of the work, cut off the power by step.

Shangyu Star Equipment Co., Ltd.

Located in Daoxu Town, Shangyu District, Shaoxing City, Zhejiang Province, it is an enterprise integrating production, product research and development, processing and trade. The company's main products: drying box series, incubator series, laboratory muffle furnace series, distilled water series, as well as clean workbench, air shower, fume hood, biological safety cabinet and other complete purification equipment.

The company has a strong technical research and development team, a strong after-sales support service system, is the leader in China's laboratory complete sets of equipment manufacturers, welcome customers to buy.