As an indispensable artifact in the laboratory, the pipette is always the "big day"!
Do you need to use it when preparing WB samples?
Do you need it when you prepare various buffers?
Do you need antibodies and sample antibodies?
The merits of your article have one tenth of it.
Assembly of the gun head:
Is "å’šå’šå’š" familiar with such a voice? If you are still struggling to put a few shots on the gun head box, put your hands on it! This can cause the internal parts of the gun (such as springs) to become loose due to the instantaneous impact force generated by the tapping, and even cause the scale adjustment knob to get stuck or even break the shot sleeve.
The correct way to do this is to insert the gun vertically into the tip of the gun and apply a slight force to the 1/4 or 1/2 turn to make it tightly fit. Multi-channel (such as 8- or 12-channel) pipetting gun, the first pipe of the pipette can be aligned with the first pipe head, then inserted obliquely, and can be clamped by shaking in the front and rear direction. The logo of the gun head is slightly over the O-ring and the connecting part can be seen to form a clear seal.
Adjustment of the range:
From large volume to small volume, rotate the knob counterclockwise according to the normal adjustment method; from small volume to large volume, first rotate the scale knob clockwise to the scale beyond the range (continue to rotate the piston button about 1/3 circle). Call back to the set volume to ensure the highest accuracy. During this process, never push the button out of the range, otherwise it will get stuck in the internal mechanism and damage the pipette.
High precision rinse:
In addition to ordinary liquid rinsing, when extracting organic solvents or highly volatile liquids, volatile gases will form a negative pressure in the white sleeve chamber, resulting in leakage. In this case, pre-washing is required 4 to 6 times. The gas in the white sleeve is saturated and the negative pressure disappears automatically.
Note: Please do not rinse the liquid at high temperature or low temperature (below freezing point)! Piston external pipettes can be considered.
Pipetting:
1. Angle of pipetting immersion: Maintain vertical position, no more than 20°. Tilting the liquid will cause a change in the pressure surface and increase the pipetting error.
2. Tip immersion depth: The correct immersion depth of the tip can effectively reduce the liquid take-off error.
3. Aspirate speed: uniform speed. The too fast aspiration speed makes it easy for the sample to form a vortex and generate bubbles; the sample liquid easily enters the sleeve and causes damage to the piston and the sealing ring; the introduction of the aerosol causes cross-contamination of the sample; especially the liquid of 1 ml or more is easy. Causes liquid to rush. After the suction, it is best to stay in the liquid surface for 1-3 seconds.
4. Draining and blowing:
Discharge: press the piston to the first discharge;
Blowing: Continue to press the piston button down to the second stop point or through the second stop point to the bottom.
5. The pipette must be removed from the gun head before it can be placed flat on the bench, as any residual liquid will flow back to the pipette when it is laid flat.
How can I tell if the pipette is in normal use?
During the experiment, we judged that the state of the pipette was found after a problem, such as air leak, sucking, or not moving, not clean; or it was obvious that it was not enough to remove 1ml of liquid. I found that there is a problem with the pipette, so in addition to the maintenance of the pipette, it is also important to do the correction yourself.
maintenance:
1. If the pipette needs to be used every day, it is recommended to clean and calibrate every three months or six months.
2. If it is not used for a long time, adjust the range of the pipette to the maximum value to keep the spring in a relaxed state to protect the spring. If you do not pay attention to this point, the piston will not rebound.
3. If the liquid accidentally enters the piston chamber, it is necessary to remove the pollutants in time.
4. Before high temperature sterilization, make sure that the pipette can adapt to high temperature, avoid deformation at higher temperature, causing leakage or inaccuracy.
5. After taking over the highly volatile and highly corrosive liquid, the whole pipette should be disassembled, the piston rod and the inner wall of the white sleeve should be rinsed with distilled water, and then installed and used after drying to avoid the volatile gas being adsorbed for a long time. The surface of the piston rod corrodes the piston rod and damages the pipette.
Shanghai Huding Bio-Friendly Reminder: Correct use of pipettes can make the experimental results more accurate, reduce experimental errors, and also extend the life of the pipette.
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